Assessing the stability of polyphenol content in red rooibos herbal tea using traditional methods and high-resolution mass spectrometry: Implications for studying dietary interventions in preclinical rodent studies.

Preclinical rodent models are used to examine the relationship between tea consumption and bone health, where tea is available for rodents and typically replaced weekly. However, the extent to which the tea polyphenols change over time remains uncertain, despite its importance in preparing tea during preclinical rodent trials. Using an untargeted molecular approach, we applied a liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-QTOFMS) system to assess the molecular profile of red rooibos teas throughout a 6-day aging period. We found a significant, 3-fold decrease of polyphenols involved in bone metabolism, including m-coumaric acid, catechin derivatives and courmaroyl tartaric acid over 6 days, likely due to photochemical decomposition and autooxidation within tea extracts. Using a novel untargeted workflow for polyphenol characterization, our findings revealed the complexity of polyphenols in red rooibos teas that can inform the evidence-based decisions of how often to change teas during in vivo rodent trials.

American College of Lifestyle Medicine Expert Consensus Statement: Lifestyle Medicine for Optimal Outcomes in Primary Care.

OBJECTIVE: Identify areas of consensus on integrating lifestyle medicine (LM) into primary care to achieve optimal outcomes. METHODS: Experts in both LM and primary care followed an a priori protocol for developing consensus statements. Using an iterative, online process, panel members expressed levels of agreement with statements, resulting in classification as consensus, near consensus, or no consensus. RESULTS: The panel identified 124 candidate statements addressing: (1) Integration into Primary Care, (2) Delivery Models, (3) Provider Education, (4) Evidence-base for LM, (5) Vital Signs, (6) Treatment, (7) Resource Referral and Reimbursement, (8) Patient, Family, and Community Involvement; Shared Decision-Making, (9) Social Determinants of Health and Health Equity, and (10) Barriers to LM. After three iterations of an online Delphi survey, statement revisions, and removal of duplicative statements, 65 statements met criteria for consensus, 24 for near consensus, and 35 for no consensus. Consensus was reached on key topics that included LM being recognized as an essential component of primary care in patients of all ages, including LM as a foundational element of health professional education. CONCLUSION: The practice of LM in primary care can be strengthened by applying these statements to improve quality of care, inform policy, and identify areas for future research.

Understanding the perspectives and needs of multiple stakeholders: Identifying key elements of a digital health intervention to protect against environmental hazards.

AirRater is a free environmental health smartphone app developed and available in Australia that collects individual health data and disseminates environmental hazard information to populations. Following previous evaluations with app users, the aim of this study was to better understand how clinicians, government agency and non-government advocacy group representatives perceive an app designed to reduce the impacts of environmental hazards on individual and public health. Nine government agency and non-government advocacy group representatives, along with 11 clinicians based in Australia participated in a semi-structured interview or focus group to explore perspectives on AirRater. Interview and focus group data were transcribed and analysed using the qualitative data analysis software NVivo. Results indicate that for clinicians, apps like AirRater can add value as an educational, patient self-management and diagnostic tool. For government and peak bodies, apps can add value by addressing environmental health literacy and monitoring and forecasting gaps, as well as supporting advocacy efforts and public health surveillance. We conclude that environmental health smartphone apps can support a range of stakeholders to achieve shared goals and priorities related to individual and public health outcomes. Further research is needed to better understand how apps could be embedded into clinical practice and policy settings.

Climate Change, Landscape Fires, and Human Health: A Global Perspective.

Landscape fires are an integral component of the Earth system and a feature of prehistoric, subsistence, and industrial economies. Specific spatiotemporal patterns of landscape fire occur in different locations around the world, shaped by the interactions between environmental and human drivers of fire activity. Seven distinct types of landscape fire emerge from these interactions: remote area fires, wildfire disasters, savanna fires, Indigenous burning, prescribed burning, agricultural burning, and deforestation fires. All can have substantial impacts on human health and well-being directly and indirectly through (a) exposure to heat flux (e.g., injuries and destructive impacts), (b) emissions (e.g., smoke-related health impacts), and (c) altered ecosystem functioning (e.g., biodiversity, amenity, water quality, and climate impacts). Minimizing the adverse effects of landscape fires on population health requires understanding how human and environmental influences on fire impacts can be modified through interventions targeted at individual, community, and regional levels. Expected final online publication date for the Annual Review of Public Health, Volume 45 is April 2024. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Pyrogeography in flux: Reorganization of Australian fire regimes in a hotter world.

Changes to the spatiotemporal patterns of wildfire are having profound implications for ecosystems and society globally, but we have limited understanding of the extent to which fire regimes will reorganize in a warming world. While predicting regime shifts remains challenging because of complex climate-vegetation-fire feedbacks, understanding the climate niches of fire regimes provides a simple way to identify locations most at risk of regime change. Using globally available satellite datasets, we constructed 14 metrics describing the spatiotemporal dimensions of fire and then delineated Australia's pyroregions-the geographic area encapsulating a broad fire regime. Cluster analysis revealed 18 pyroregions, notably including the (1) high-intensity, infrequent fires of the temperate forests, (2) high-frequency, smaller fires of the tropical savanna, and (3) low-intensity, diurnal, human-engineered fires of the agricultural zones. To inform the risk of regime shifts, we identified locations where the climate under three CMIP6 scenarios is projected to shift (i) beyond each pyroregion's historical climate niche, and (ii) into climate space that is novel to the Australian continent. Under middle-of-the-road climate projections (SSP2-4.5), an average of 65% of the extent of the pyroregions occurred beyond their historical climate niches by 2081-2100. Further, 52% of pyroregion extents, on average, were projected to occur in climate space without present-day analogues on the Australian continent, implying high risk of shifting to states that also lack present-day counterparts. Pyroregions in tropical and hot-arid climates were most at risk of shifting into both locally and continentally novel climate space because (i) their niches are narrower than southern temperate pyroregions, and (ii) their already-hot climates lead to earlier departure from present-day climate space. Such a shift implies widespread risk of regime shifts and the emergence of no-analogue fire regimes. Our approach can be applied to other regions to assess vulnerability to rapid fire regime change.

Synthesis of a photocleavable bola-phosphatidylcholine.

Phosphatidylinositol transfer proteins (PITPs) are ubiquitous in eukaryotes and are involved in the regulation of phospholipid metabolism, membrane trafficking, and signal transduction. Sec14 is a yeast PITP that has been shown to transfer phosphatidylinositol (PI) or phosphatidylcholine (PC) from the endoplasmic reticulum to the Golgi. It is now believed that Sec14 may play a greater role than just shuttling PI and PC throughout the cell. Genetic evidence suggests that retrieval of membrane-bound PI by Sec14 also manages to present PI to the phosphatidylinositol-4-kinase, Pik1, to generate phosphatidylinositol-4-phosphate, PI(4)P. To test this hypothetical model, we designed a photocleavable bolalipid to span the entire membrane, having one phosphatidylcholine or phosphatidylinositol headgroup on each leaflet connected by a photocleavable diacid. Sec14 should not be able to present the bola-PI to Pik1 for phosphorylation as the head group will be difficult to lift from the bilayer as it is tethered on the opposite leaflet. After photocleavage the two halves would behave as a normal phospholipid, thus phosphorylation by Pik1 would resume. We report here the synthesis of a photocleavable bola-PC, a precursor to the desired bola-PI. The mono-photocleavable bola-PC lipid was designed to contain two glycerol molecules with choline head groups connected through a phosphodiester bond at the sn3 position. Each glycerol was acylated with palmitic acid at the sn1 position. These two glycerol moieties were then connected through their respective sn2 hydroxyls via a photocleavable dicarboxylic acid containing a nitrophenyl ethyl photolabile protecting group. The bola-PC and its precursors were found to undergo efficient photocleavage when irradiated in solution or in vesicles with 365 nm light for two minutes. Treatment of the bola-PC with a mutant phospholipase D and myo-inositol produced a mono-inositol bola-PC-PI.

HEALing Our Nation - Health Equity Achieved Through Lifestyle Medicine Capturing Highlights From the "HEALing Our Nation" Session at LM2023 and Outlining the Important Work of the HEAL Initiative.

Since the COVID-19 pandemic, health equity has been placed front and center in the conversations surrounding healthcare as well other fields. This conversation has also been occurring in the field of lifestyle medicine with an intentional focus on developing solutions at the intersection of lifestyle medicine and health equity. Initiated by a call to action by ACLM Past President Dexter Shurney at the 2019 Lifestyle Medicine conference, the HEAL Initiative was created with that intention, to address health disparities and advance health equity through lifestyle medicine. Since 2019, the HEAL initiative has grown considerably in its work and impact, creating solutions aligned with the AMA strategic planning recommendations as well developing projects that are examples of community engaged-lifestyle medicine. The work of the HEAL initiative culminated in a full circle moment at the 2023 Lifestyle Medicine Conference which featured an interview (facilitated by Dr. Dexter Shurney) with former US Surgeon General Dr. Jerome Adams and review of HEAL's work over the past 3 years. This article will capture the key highlights of the HEALing our Nation opening session and the cumulative work of HEAL Initiative.

Taming the flame, from local to global extreme wildfires.

Australia rethinks strategies after 2019 to 2020 bushfires.

Wildfire national carbon accounting: how natural and anthropogenic landscape fires emissions are treated in the 2020 Australian government greenhouse gas accounts report to the UNFCCC.

Greenhouse gas (GHG) accounting of emissions from land use, land-use change, and forestry necessarily involves consideration of landscape fire. This is of particular importance for Australia given that natural and human fire is a common occurrence, and many ecosystems are adapted to fire, and require periodic burning for plant regeneration and ecological health. Landscape fire takes many forms, can be started by humans or by lightning, and can be managed or uncontrolled. We briefly review the underlying logic of greenhouse gas accounting involving landscape fire in the 2020 Australian Government GHG inventory report. The treatment of wildfire that Australia chooses to enact under the internationally agreed guidelines is based on two core assumptions (a) that effects of natural and anthropogenic fire in Australian vegetation carbon stocks are transient and they return to the pre-fire level relatively quickly, and (b) that historically and geographically anomalous wildfires in forests should be excluded from national anthropogenic emission estimates because they are beyond human control. It is now widely accepted that anthropogenic climate change is contributing to increased frequency and severity of forest fires in Australia, therefore challenging assumptions about the human agency in fire-related GHG emissions and carbon balance. Currently, the national inventory focuses on forest fires; we suggest national greenhouse gas accounting needs to provide a more detailed reporting of vegetation fires including: (a) more detailed mapping of fire severity patterns; (b) more comprehensive emission factors; (c) better growth and recovery models from different vegetation types; (d) improved understanding how fires of different severities affect carbon stocks; and (e) improved analysis of the human agency behind the causes of emissions, including ignition types and fire-weather conditions. This more comprehensive accounting of carbon emissions would provide greater incentives to improve fire management practices that reduce the frequency, severity, and extent of uncontrolled landscape fires.

Mechanical treatments and prescribed burning can reintroduce low-severity fire in southern Australian temperate sclerophyll forests.

The establishment of sustainable, low-intensity fire regimes is a pressing global challenge given escalating risk of wildfire driven by climate change. Globally, colonialism and industrialisation have disrupted traditional fire management, such as Indigenous patch burning and silvo-pastoral practices, leading to substantial build-up of fuel and increased fire risk. The disruption of fire regimes in southeastern Tasmania has led to dense even-aged regrowth in wet forests that are prone to crown fires, and dense Allocasuarina-dominated understoreys in dry forests that burn at high intensities. Here, we investigated the effectiveness of several fire management interventions at reducing fire risk. These interventions involved prescribed burning or mechanical understorey removal techniques. We focused on wet and dry Eucalyptus-dominated sclerophyll forests on the slopes of kunanyi/Mt. Wellington in Hobart, Tasmania, Australia. We modelled potential fire behaviour in these treated wet and dry forests using fire behaviour equations based on measurements of fuel load, vegetation structure, understorey microclimate and regional meteorological data. We found that (a) fuel treatments were effective in wet and dry forests in reducing fuel load, though each targeted different layers, (b) both mechanical treatments and prescribed burning resulted in slightly drier, and hence more fire prone understorey microclimate, and (c) all treatments reduced predicted subsequent fire severity by roughly 2-4 fold. Our results highlight the importance of reducing fuel loads, even though fuel treatments make forest microclimates drier, and hence fuel more flammable. Our finding of the effectiveness of mechanical treatments in lowering fire risk enables managers to reduce fuels without the risk of uncontrolled fires and smoke pollution that is associated with prescribed burning. Understanding the economic and ecological costs and benefits of mechanic treatment compared to prescribed burning requires further research.

Fire intensity impacts on physiological performance and mortality in Pinus monticola and Pseudotsuga menziesii saplings: a dose-response analysis.

Fire is a major cause of tree injury and mortality worldwide, yet our current understanding of fire effects is largely based on ocular estimates of stem charring and foliage discoloration, which are error prone and provide little information on underlying tree function. Accurate quantification of physiological performance is a research and forest management need, given that declining performance could help identify mechanisms of-and serve as an early warning sign for-mortality. Many previous efforts have been hampered by the inability to quantify the heat flux that a tree experiences during a fire, given its highly variable nature in space and time. In this study, we used a dose-response approach to elucidate fire impacts by subjecting Pinus monticola var. minima Lemmon and Pseudotsuga menziesii (Mirb.) Franco var. glauca (Beissn.) Franco saplings to surface fires of varying intensity doses and measuring short-term post-fire physiological performance in photosynthetic rate and chlorophyll fluorescence. We also evaluated the ability of spectral reflectance indices to quantify change in physiological performance at the individual tree crown and stand scales. Although physiological performance in both P. monticola and P. menziesii declined with increasing fire intensity, P. monticola maintained a greater photosynthetic rate and higher chlorophyll fluorescence at higher doses, for longer after the fire. Pinus monticola also had complete survival at lower fire intensity doses, whereas P. menziesii had some mortality at all doses, implying higher fire resistance for P. monticola at this life stage. Generally, individual-scale spectral indices were more accurate at quantifying physiological performance than those acquired at the stand-scale. The Photochemical Reflectance Index outperformed other indices at quantifying photosynthesis and chlorophyll fluorescence, highlighting its potential use to quantify crown scale physiological performance. Spectral indices that incorporated near-infrared and shortwave infrared reflectance, such as the Normalized Burn Ratio, were accurate at characterizing stand-scale mortality. The results from this study were included in a conifer cross-comparison using physiology and mortality data from other dose-response studies. The comparison highlights the close evolutionary relationship between fire and species within the Pinus genus, assessed to date, given the high survivorship of Pinus species at lower fire intensities versus other conifers.

Evaluating User Preferences, Comprehension, and Trust in Apps for Environmental Health Hazards: Qualitative Case Study.

BACKGROUND: Climate change is projected to increase environmental health hazard risks through fire-related air pollution and increased airborne pollen levels. To protect vulnerable populations, it is imperative that evidence-based and accessible interventions are available. The environmental health app, AirRater, was developed in 2015 in Australia to provide information on multiple atmospheric health hazards in near real time. The app allows users to view local environmental conditions, and input and track their personal symptoms to enable behaviors that protect health in response to environmental hazards. OBJECTIVE: This study aimed to develop insights into users' perceptions of engagement, comprehension, and trust in AirRater to inform the future development of environmental health apps. Specifically, this study explored which AirRater features users engaged with, what additional features or functionality needs users felt they required, users' self-perception of understanding app information, and their level of trust in the information provided. METHODS: A total of 42 adult AirRater users were recruited from 3 locations in Australia to participate in semistructured interviews to capture location- or context-specific experiences. Participants were notified of the recruitment opportunity through multiple avenues including newsletter articles and social media. Informed consent was obtained before participation, and the participants were remunerated for their time and perspectives. A preinterview questionnaire collected data including age range, any preexisting conditions, and location (postcode). All participant data were deidentified. Interviews were recorded, transcribed, and analyzed using thematic analysis in NVivo 12 (QSR International). RESULTS: Participants discussed app features and functionality, as well as their understanding of, and trust in, the information provided by the app. Most (26/42, 62%) participants used and valued visual environmental hazard features, especially maps, location settings, and hazard alerts. Most (33/42, 78%) found information in the app easy to understand and support their needs, irrespective of their self-reported literacy levels. Many (21/42, 50%) users reported that they did not question the accuracy of the data presented in the app. Suggested enhancements include the provision of meteorological information (eg, wind speed or direction, air pressure, UV rating, and humidity), functionality enhancements (eg, forecasting, additional alerts, and the inclusion of health advice), and clarification of existing information (eg, symptom triggers), including the capacity to download personal summary data for a specified period. CONCLUSIONS: Participants' perspectives can inform the future development of environmental health apps. Specifically, participants' insights support the identification of key elements for the optimal development of environmental health app design, including streamlining, capacity for users to customize, use of real time data, visual cues, credibility, and accuracy of data. The results also suggest that, in the future, iterative collaboration between developers, environmental agencies, and users will likely promote better functional design, user trust in the data, and ultimately better population health outcomes.

Population collapse of a Gondwanan conifer follows the loss of Indigenous fire regimes in a northern Australian savanna.

Colonialism has disrupted Indigenous socioecological systems around the globe, including those supported by intentional landscape burning. Because most disruptions happened centuries ago, our understanding of Indigenous fire management is largely inferential and open to debate. Here, we investigate the ecological consequences of the loss of traditional Aboriginal fire management on fire-exposed savannas on the Arnhem Plateau, northern Australia, using the fire-sensitive conifer Callitris intratropica as a bio-indicator. We contrast Kakadu National Park, where traditional Aboriginal fire management was severely disrupted during the early twentieth century following Aboriginal relocation to surrounding settlements, and an adjacent Aboriginal estate where traditional Aboriginal fire management endures. Since 2006, traditional Aboriginal fire management at this site has been overlaid by a program of broad-scale institutionalized burning in the early dry season, designed to reduce greenhouse emissions. Using remote sensing, field survey, and dendrochronology, we show that on the Aboriginal estate, C. intratropica populations depend on the creation of a shifting patch mosaic of long unburned areas necessary for the recruitment of C. intratropica. However, the imposition of broad-scale fire management is disrupting this population patch dynamic. In Kakadu, there have been extreme declines of C. intratropica associated with widespread fires since the mid twentieth century and consequent proliferation of grass fuels. Fire management in Kakadu since 2007, designed to increase the size and abundance of patches of unburned vegetation, has not been able to reverse the population collapse of C. intratropica. Our study demonstrates that colonial processes including relocation of Indigenous people and institutional fire management can have deleterious consequences that are nearly irreversible because of hysteresis in C. intratropica population dynamics.

Carbon dioxide and particulate emissions from the 2013 Tasmanian firestorm: implications for Australian carbon accounting.

BACKGROUND: Uncontrolled wildfires in Australian temperate Eucalyptus forests produce significant smoke emissions, particularly carbon dioxide (CO2) and particulates. Emissions from fires in these ecosystems, however, have received less research attention than the fires in North American conifer forests or frequently burned Australian tropical savannas. Here, we use the 2013 Forcett-Dunalley fire that caused the first recorded pyrocumulonimbus event in Tasmania, to understand CO2 and particulate matter (PM2.5) emissions from a severe Eucalyptus forest fire. We investigate the spatial patterns of the two emissions using a fine scale mapping of vegetation and fire severity (50 m resolution), and utilising available emission factors suitable for Australian vegetation types. We compare the results with coarse-scale (28 km resolution) emissions estimates from Global Fire Emissions Database (GFED) to determine the reliability of the global model in emissions estimation. RESULTS: The fine scale inventory yielded total CO2 emission of 1.125 ± 0.232 Tg and PM2.5 emission of 0.022 ± 0.006 Tg, representing a loss of 56 t CO2 ha-1 and 1 t PM2.5 ha-1. The CO2 emissions were comparable to GFED estimates, but GFED PM2.5 estimates were lower by a factor of three. This study highlights the reliability of GFED for CO2 but not PM2.5 for estimating emissions from Eucalyptus forest fires. Our fine scale and GFED estimates showed that the Forcett-Dunalley fire produced 30% of 2013 fire carbon emissions in Tasmania, and 26-36% of mean annual fire emissions for the State, representing a significant single source of emissions. CONCLUSIONS: Our analyses highlight the need for improved PM2.5 emission factors specific to Australian vegetation, and better characterisation of fuel loads, particularly coarse fuel loads, to quantify wildfire particulate and greenhouse gas emissions more accurately. Current Australian carbon accountancy approach of excluding large wildfires from final GHG accounts likely exaggerates Tasmania's claim to carbon neutrality; we therefore recommend that planned and unplanned emissions are included in the final national and state greenhouse gas accounting to international conventions. Advancing these issues is important given the trajectory of more frequent large fires driven by anthropogenic climate change.

Smoke pollution must be part of the savanna fire management equation: A case study from Darwin, Australia.

Savanna fire management is a topic of global debate, with early dry season burning promoted as a large-scale emissions reduction opportunity. To date, discussions have centred on carbon abatement efficacy, biodiversity and cultural benefits and/or risks. Here we use a case study of Darwin, Australia to highlight smoke pollution as another critical consideration. Smoke pollution from savanna fires is a major public health issue, yet absent so far from discussions of program design. Here, we assess the likely impacts of increased early dry season burning on smoke pollution in Darwin between 2004 and 2019, spanning the introduction and expansion of carbon abatement programs. We found increased smoke pollution in the early dry season but little change in the late dry season, contributing to a net annual increase in air quality standard exceedances. Geospatial analysis suggests this relates to increased burning in the path of early dry season trade winds. This study highlights the complex health trade-offs involved with any large-scale prescribed burning, including for carbon abatement.

Thin-film microextraction combined with comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry screening for presence of multiclass organic pollutants in drinking water samples.

Organic compounds in drinking water can be potentially hazardous. In this study, we demonstrate for the first time that combining in-bottle thin-film microextraction (TFME), comprehensive two-dimensional gas chromatography, and time-of-flight mass spectrometry (GC × GC-TOFMS) can produce a powerful, green tool for determining multiple organic pollutants in drinking water and monitoring changes in water samples after treatment with a water purification system. In the developed approach, water is added to an amber sampling bottle containing TFME membranes and left to sit until equilibration is achieved. Once equilibration has been achieved, the membranes are withdrawn, and the extracted compounds are quantified via thermal desorption in the GC × GC-TOFMS system. This approach enabled a large number of organic compounds with a wide range of physicochemical properties to be identified based on their mass spectra. A series of drinking water samples (raw water, water after softener filter, and drinking water) were collected from a standard house faucet in order to analyze changes following treatment with a water purification system. The developed strategy was also applied to identify the concentrations of the 9 selected organic compounds in the water samples. Ultimately, the in-bottle TFME-GC × GC-TOFMS method is straightforward and provides comparable performance to other methods for low-level analyses of organic pollutants in drinking water samples.

Global increase in wildfire risk due to climate-driven declines in fuel moisture.

There is mounting concern that global wildfire activity is shifting in frequency, intensity, and seasonality in response to climate change. Fuel moisture provides a powerful means of detecting changing fire potential. Here, we use global burned area, weather reanalysis data, and the Canadian fire weather index system to calculate fuel moisture trends for multiscale biogeographic regions across a gradient in vegetation productivity. We quantify the proportion of days in the local fire season between 1979 and 2019, where fuel moisture content is below a critical threshold indicating extreme fire potential. We then associate fuel moisture trends over that period to vegetation productivity and comment on its implications for projected anthropogenic climate change. Overall, there is a strong drying trend across realms, biomes, and the productivity gradient. Even where a wetting trend is observed, this often indicates a trend toward increasing fire activity due to an expected increase in fuel production. The detected trends across the productivity gradient lead us to conclude global fire activity will increase with anthropogenic climate change.

AusTraits, a curated plant trait database for the Australian flora.

We introduce the AusTraits database - a compilation of values of plant traits for taxa in the Australian flora (hereafter AusTraits). AusTraits synthesises data on 448 traits across 28,640 taxa from field campaigns, published literature, taxonomic monographs, and individual taxon descriptions. Traits vary in scope from physiological measures of performance (e.g. photosynthetic gas exchange, water-use efficiency) to morphological attributes (e.g. leaf area, seed mass, plant height) which link to aspects of ecological variation. AusTraits contains curated and harmonised individual- and species-level measurements coupled to, where available, contextual information on site properties and experimental conditions. This article provides information on version 3.0.2 of AusTraits which contains data for 997,808 trait-by-taxon combinations. We envision AusTraits as an ongoing collaborative initiative for easily archiving and sharing trait data, which also provides a template for other national or regional initiatives globally to fill persistent gaps in trait knowledge.